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Gel Electrophoresis can separate macromolecules (DNA/RNA, protein) based on their size and charge. Larger molecules move slower through gel matrix), and ones with less negative charge would move slowly towards the positive electrode. The smallest molecules travel furthest but secondary and tertiary structures also affect mobility. Molecules can be denatured which alters secondary structure so only molecular size assessed.
|Western blot||Protein e.g. for haemoglobin is a protein. For sickle cell mutation (glutamate to lysine). An increased positive charge results with change in behaviour on blot|